The Utility of Trouble: Maximizing the Value of Our Human Services Dollars

Outlines recommendations to standardize service delivery areas and consolidate area offices of the state's seven largest human services agencies, as well as to close antiquated institutions. Projects benefits such as improved accessibility and savings

Monitoring of Carboxypeptidase Digestion by Matrix-Assisted Laser Desorption and Ionization Mass Spectrometry

The potential of matrix-assisted laser desorption and ionization mass spectrometry (LDI-MS) is demonstrated by monitoring and analyzing the digestion of (human) pTH (1–34), a synthetic peptide with carboxypeptidases Y and B. All occurring ion signals in the mass spectra could be identified as degraded peptides. By calculating the mass differences between successive degraded peptides, it was possible to identify the released amino acids and to determine 8 amino acids of the C-terminus of the original peptide. For a single MS measurement, only 2 pmol of substrate was needed. Time-course analysis of the cleavage of the first amino acid residue gave insight into the kinetics involved. These measurements strongly support the hope that quantitative information about concentrations can be extracted from LDI-MS

SEROLOGICALLY DEFINED AND LYMPHOCYTE-DEFINED COMPONENTS OF THE MAJOR HISTOCOMPATIBILITY COMPLEX IN THE MOUSE

The mixed leukocyte culture (MLC) test is an in vitro model of the recognition phase of the homograft response. For the most part, activation in MLC is dependent on differences of the major histocompatibility complex (MHC). Our present studies in the mouse suggest that activation is primarily associated with differences of genetic regions of the MHC other than those which control the serologically defined (H-2) antigens. These differences do not lead to cytotoxic or agglutinating antibody formation despite extensive immunization; we have called these differences lymphocyte-defined (LD) differences. The strongest stimulation in MLC is associated with differences of the Ir region. It is possible that the Ir product is the T cell receptor and that it is this same molecule which can act as the stimulatory agent in MLC. Other possibilities are discussed

Importance of Different Regions of H-2 for MLC Stimulation 1

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/65829/1/j.1399-0039.1973.tb01008.x.pd

Miniaturization of Chemical Analysis Systems – A Look into Next Century's Technology or Just a Fashionable Craze?

Miniaturization of already existing techniques in on-line analytical chemistry is an alternative to compound-selective chemical sensors. Theory points in the direction of higher efficiency, faster analysis time, and lower reagent consumption. Micromachining, a well known photolithographic technique for structures in the micrometer range, is introduced and documented with structures as examples for flow injection analysis, electrophoresis, and a detector cell

Mixed lymphocyte culture reactivity and H-2 histocompatibility loci differences,

Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/34061/1/0000339.pd